The incidence of infections caused by â-lactam resistant bacteria due to the production of Extended
Spectrum Beta-lactamase (ESBL) enzyme is on the increase both in hospital and community settings
with the associated high morbidity and mortality. This study aimed at determining the prevalence of
ESBL-producing Gram negative clinical isolates from three General Hospitals in Lagos, Nigeria. A
total of 147 Gram-negative bacilli bacteria isolated from clinical samples were identified using
biochemical tests. Susceptibility to ten antibiotics was carried out using standard disc diffusion method
and phenotypic detection of ESBL production was done using Double Disk Synergy Test (DDST).
Antimicrobial susceptibility test showed 80(54.4%), 86(58.5%), 73(49.7%), 55(37.4%), 74(50.3%),
47(32.0%),44(29.9%),60(40.8%),33(22.4%)and12(8.2%)resistancetoSulphamethoxazole/Trimethoprim,
Cefotaxime,Aztreonam, Ciprofloxacin, Ceftriaxone, Ceftazidime, Cefepime, Cefoxitin, Gentamicin and
Imipenem, respectively. One hundred and fourteen (77.6%) of the isolates were found to be resistant to
one or more â-lactams, and ESBL production was detected in 29.8% (34/114) of them. The overall
prevalence of ESBLproducing isolates in this study was 23.1%(34/147); and there was no significant
difference in the prevalence among the hospitals. with highest number occurring in Escherichia coli
(12/35). Resistance to eight out of the ten antibiotics was significantly higher (p<0.05) among ESBL
producers, with co-resistance to ciprofloxacin, gentamicin and cotrimoxazole compared to non-ESBL
producers. This study confirms the occurrence of multidrug resistant ESBLproducing clinical isolates
in three General Hospitals in Lagos, which is of serious public health significance, hence proper
diagnosis and treatment methods should be adopted to control the spread of these strains.
- Obika, O. A., Adekanye, A. A. and Sonaike, T. B.
- E.A. ADEMOLA Article-65a151b9
E.A. ADEMOLA Article-65a151b9